Prolific forward was making a rare appearance for his country against the Irish.
England will be without in-form front man Daniel Sturridge through injury when they travel to Brazil to play the five-time world champions in a friendly international on Sunday, reports BBC Sport Online.
The Liverpool attacker was making his first-ever start under new Three Lions head coach Roy Hodgson against the Republic of Ireland at Wembley Stadium on Wednesday night.
However, after creating the home side’s first-half equaliser for midfield player Frank Lampard, the 23-year-old was then stretchered off with an ankle injury shortly afterwards.
And after the match had ended in a 1-1 draw, Hodgson confirmed his and Liverpool’s worst fears that Sturridge had in fact suffered ankle ligament damage that will rule the striker out for a number of weeks.
“He’s not going to Brazil,” said Hodgson. “He had a good start and we were sad to lose him.
“He hadn’t played for a while and was carrying a slight injury. I fear it will keep him out for a few weeks.”
Sturridge has appeared for his country on five occasions since making his England debut two years ago, finding the back of the net just once in that time.